Effect of substrate on biochemical expression of Bacterial Biofilms

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dc.contributor.author Lakmali, N.A.D.
dc.contributor.author Singhalage, I.D.
dc.contributor.author Ediriweera, E.P.S.K.
dc.contributor.author Senevirathne, G.
dc.date.accessioned 2022-02-15T06:29:40Z
dc.date.available 2022-02-15T06:29:40Z
dc.date.issued 2015
dc.identifier.isbn 9789550481088
dc.identifier.uri http://www.erepo.lib.uwu.ac.lk/bitstream/handle/123456789/8353/47-SCT-Effect%20of%20substrate%20on%20biochemical%20expression%20of%20Bacterial%20Biofilms%20.pdf?sequence=1&isAllowed=y
dc.description.abstract Single or multi species bacteria are agglomerated together with the help of Extracellular Polymeric Substance (EPS) forming bacterial biofilms (BBs). BBs get attached to the biotic or abiotic surfaces. This surface based adhesion alters the biochemical expression of BBs. Under the optimum conditions, the biochemical expression of BBs can be maximized. The BBs can be used in various biotechnological applications like biofertilizers, drug discovery, biofuels etc. (Senaviratne, et al., 2008). Thus, present study figure outs preferable surface for the BBs, which would enhance the functional properties of BBs. Methodology In this experiment, single species bacterial biofilms were formed in glass wool (G), metal fibers (M) and fungal filaments (F) immersed in a Low Cost Medium (LCM). Control (C) was maintained without surfaces or bacterial strain. There were three replicates for each treatment. Completely Randomized Design was followed as the experimental design. Attachment of bacterial biofilm with different surfaces was observed through the microscopy. Biochemical expressions of different biofilms were examined by analyzing the exudates using Fourier Transform Infrared (FTIR) print, polysaccharide, amide, fatty acid I, II, and mixed. Cluster analysis was performed under each window to see the similarity and the differences of the biochemical expressions of bacterial biofilms attached with different surfaces. Statistical analysis was done by using Minitab statistical package (version 16). en_US
dc.language.iso en en_US
dc.publisher Uva Wellassa University of Sri Lanka en_US
dc.subject Science and Technology en_US
dc.subject Technology en_US
dc.subject Biochemistry en_US
dc.subject Biofilms en_US
dc.subject Chemical Engineering en_US
dc.title Effect of substrate on biochemical expression of Bacterial Biofilms en_US
dc.title.alternative Research Symposium 2015 en_US
dc.type Other en_US

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