Abstract:
Single or multi species bacteria are agglomerated together with the help of Extracellular Polymeric
Substance (EPS) forming bacterial biofilms (BBs). BBs get attached to the biotic or abiotic surfaces.
This surface based adhesion alters the biochemical expression of BBs. Under the optimum
conditions, the biochemical expression of BBs can be maximized. The BBs can be used in various
biotechnological applications like biofertilizers, drug discovery, biofuels etc. (Senaviratne, et al.,
2008). Thus, present study figure outs preferable surface for the BBs, which would enhance the
functional properties of BBs.
Methodology
In this experiment, single species bacterial biofilms were formed in glass wool (G), metal fibers (M)
and fungal filaments (F) immersed in a Low Cost Medium (LCM). Control (C) was maintained
without surfaces or bacterial strain. There were three replicates for each treatment. Completely
Randomized Design was followed as the experimental design. Attachment of bacterial biofilm with
different surfaces was observed through the microscopy. Biochemical expressions of different
biofilms were examined by analyzing the exudates using Fourier Transform Infrared (FTIR)
print,
polysaccharide, amide, fatty acid I, II, and mixed. Cluster analysis was performed under each window
to see the similarity and the differences of the biochemical expressions of bacterial biofilms attached
with different surfaces. Statistical analysis was done by using Minitab statistical package (version
16).
