Variation of Mesophilic and Thermophilic Spore Counts in Uht Processing of Full Cream Milk

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dc.contributor.author SENEVIRATNE, A.W.D.T.
dc.date.accessioned 2021-10-12T08:31:13Z
dc.date.available 2021-10-12T08:31:13Z
dc.date.issued 2014
dc.identifier.other UWU/ANS/14/0038
dc.identifier.uri http://www.erepo.lib.uwu.ac.lk/bitstream/handle/123456789/7251/ANS%2014%20038-17022021134201.pdf?sequence=1&isAllowed=y
dc.description.abstract Demand for the UHT treated milk has been largely increased in the Sri Lankan market in the recent past. Quality deterioration of UHT products will degrade the brand reputation and make health risks for the consumers. Thus, the present study was focused to determine mesophilic (MP) and thermophilic (TP) spore count variation pattern in UHT processing of full cream milk and to identify contamination points in the processing line. Six control points in the processing line were identified; (milk receiving bowsers, milk line-just after thermizing, silos-6-12h after storage, processing line-just after pasteurizing the mix, mix storage tanks (MST)-1h after pasteurized mix stored, final product-soon after UHT treatment) and 16 milk samples from each point were collected in triplicates. Spore counts were evaluated by heating to 100°C for 10 minutes and subsequent culturing on plate count agar followed by incubation at 55°C for 2 days for TP spore counts and at 35°C for MP spore counts. Vegetative cell counts and spore counts were evaluated during 4 months of ambient temperature storage at 30 days interval. Swab samples and clean- in- place final rinse samples were collected from 6 control points and analyzed for Coliforms and total plate count. Significantly higher MP and TP spore counts were observed as 9.00±8.79x102 cfu/mL and 1.09±1.01x 102cfu/mL, respectively in MST after pasteurization. This may be due to the heat stress at the pasteurization process which may have induced the spore formation by bacteria. Significantly lower MP and TP counts were observed in the final 'product (1.70±5.30cfu/mL and 3.34±17.9x10-2cfu/mL respectively). In conclusion, there is a requirement to initiate good agricultural practices to reduce bacterial load coming from the farm level and to initiate good manufacturing practices to reduce the cross contamination during processing. Key words: Mesophilic spores, Thermophilic spores, 'UHT processing en_US
dc.language.iso en en_US
dc.publisher Uva Wellassa University of Sri Lanka en_US
dc.relation.ispartofseries ;UWU/ANS/14/0038
dc.subject Animal Science Degree Programme (ANS) en_US
dc.title Variation of Mesophilic and Thermophilic Spore Counts in Uht Processing of Full Cream Milk en_US
dc.title.alternative Research Article – ANS 2014 en_US
dc.type Thesis en_US


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