PROTOCOL FOR CALLUS INDUCTION OF Camellia japonica L. (TEA ROSE)

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dc.contributor.author DILRUKSHIKA, H.L.T.
dc.date.accessioned 2021-08-19T07:45:25Z
dc.date.available 2021-08-19T07:45:25Z
dc.date.issued 2014
dc.identifier.other UWU/TEA/10/0007
dc.identifier.uri http://www.erepo.lib.uwu.ac.lk/bitstream/handle/123456789/6966/UWULD%20TEA%2010%200007-03052019153551.pdf?sequence=1&isAllowed=y
dc.description.abstract Camellia japonica L. is one of the best known species of the genus Camellia and considered as a kind of famous ornamental flower. This study was conduct to develop an efficient protocol for rapid and prolific callus induction of Camellia japonica (Tea Rose). Leaves, nodal segments and unopened flower buds flower petals were used as explants. Nine different combinations of 20% sodium hypochlorite and 70% ethanol with different soaking times were used to select the best sterilization method. The sterilized explants were cultured on MS medium with three different hormone combinations of 3-indolebutric acid (IBA) and 6-benzylamino purine (BAP) to investigate the effect on callus induction. Cultures were maintained at 25+2 °C temperature under completely dark conditions in an incubator. Semi mature light green (1 cm2) pieces of leaves sterilized using 20% sodium hypochlorite for 40 minutes and 70% ethanol for 60 seconds and pieces of semi mature light brown (1cm) nodal segments using 20% sodium hypochlorite for 45 minutes and 70% ethanol for 90 seconds showed pronounced effect which resulted 20% and 30% of contaminations respectively after one week of culturing. MS supplemented with 0.9% agar, 3% sucrose, 0.001% myo-inositol, with lmg/L IBA and 2mg/L BAP was recorded better hormone combination for callus formation from Camellia japonica nodal segments within 19 days and MS supplemented with 0.9% agar, 3% sucrose, 0.001% myo-inositol, with lmg/L IBA and 4mg/L BAP was recorded the better hormone combination for callus formation from pieces of Camellia japonica leaves within 25 days. Nodal callus showed watery, soft and light yellow in color and globular shape and leaf callus showed friable and greenish yellow in color. As a protocol for callus induction of C. japonica using leaves and nodal segments, above methods of surface sterilization and culture establishment can be used successfully. en_US
dc.language.iso en en_US
dc.publisher Uva Wellassa University of Sri Lanka en_US
dc.relation.ispartofseries ;UWU/TEA/10/0007
dc.subject Tea Technology and Value Addition Degree Programme ( TEA) en_US
dc.title PROTOCOL FOR CALLUS INDUCTION OF Camellia japonica L. (TEA ROSE) en_US
dc.title.alternative Research Article – TEA 2014 en_US
dc.type Thesis en_US


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