Identification of Vectors for In-vivo Protein Overexpression in Introducing Drought Resistance: A Review

Show simple item record Cooray, R. Dissanayake, Y. Warnakula, L. Hapuarachchi, N.S. 2019-05-08T04:59:47Z 2019-05-08T04:59:47Z 2019
dc.identifier.isbn 9789550481255
dc.description.abstract The prolonged lack of rainfall is a major abiotic stress affecting crop productivity in Sri Lanka. In vivo protein overexpression to induce drought resistance is frequently studied globally however; the selection of a most suitable vector for gene transfection has been a challenge for high protein yield. This study aims to review such possible vectors, through a thorough published literature survey at ‘Google scholar’ and ‘PubMed’ with search terms ‘drought resist*’, ‘drought tolerant*’, ‘vector’, ‘overexpression’. A late embryogenesis abundant protein gene OsLEA3-1 overexpressed with three binary expression constructs (OsLEA3-S/A/H), by inserting the full length cDNA into backbone vectors pCAMBIA1301-S/A/H respectively, with double CaMV 35S, rice Actin1 and HVA1-like promoter, followed by Agrobacterium transformation. All other constructs OsLEA3-S/-H had higher grain yield than wild type under stress except OsLEA3-A. Over expression levels are 63% 56% and 46% for OsLEA3-S/A/H, respectively. Protein coding region of OsbZIP72 in vector pCAMBIA1300S to construct pCAMBIA1300S-OsbZIP72; and a promoter of drought tolerance rice cultivar IRAT109 in a pCAMBIA1381xb-GFP vector to control GFP expression and Arabidopsis BnLEA4-1 in pGEMT-BnLEA4-1 vector transfected via Agrobacterium are few examples at global scale. However, no published studies in Sri Lanka were found during the literature search. Therefore, cloning of drought tolerant gene to a crop plant followed by in vivo overexpression to stimulate the drought tolerance is a promising biotechnological advancement in agriculture. Literature showed vectors can be modified to fit to an individual crop product for in vivo overexpression of drought tolerant genes. It is recommended to extend the review to analyse characteristics for a suitable vector include compatibility, linker region, and screening genes to develop a complete guide for selection of vector in in vivo protein overexpression for drought resistance. en_US
dc.language.iso en en_US
dc.publisher Uva Wellassa University of Sri Lanka en_US
dc.subject Genetics en_US
dc.subject Biotechnology en_US
dc.title Identification of Vectors for In-vivo Protein Overexpression in Introducing Drought Resistance: A Review en_US
dc.title.alternative International Research Conference 2019 en_US
dc.type Other en_US

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