Comparison of ABTS, DPPH, and FRAP Assays for Estimating Antioxidant Potential of Selected Sri Lankan Traditional Sweetmeats

Show simple item record

dc.contributor.author Mihiranie, M.K.S.
dc.contributor.author Jayasinghe, J.M.J.K.
dc.contributor.author Wanasundara, J.P.D.
dc.contributor.author Jayasinghe, C.V.L.
dc.date.accessioned 2019-05-07T04:48:29Z
dc.date.available 2019-05-07T04:48:29Z
dc.date.issued 2019
dc.identifier.isbn 9789550481255
dc.identifier.uri http://www.erepo.lib.uwu.ac.lk/handle/123456789/459
dc.description.abstract Fifteen Sri Lankan traditional sweetmeats (Hendi kewum, Beraliya kewum, Naran kewum, Athirasa, Mung kewum, Aasmi, Kokis, Undu walalu, Welithalapa, Bedihaalpiti aggala, Dodol, Aluwa, Thala guli, Kurahan helapa and Pusnambu) were investigated for antioxidant potential (AP) by 2,2-diphenyl-1- picrylhydrazyl assay (DPPH), 2,2-azino-bis-3-ethylbenzothiazoline-6-sulphonic acid (ABTS) assay and Ferrous reducing antioxidant power assay (FRAP). Total phenolic content (TPC) and total flavonoid content (TFC) of sweetmeats were determined by using colorimetric assays. Lyophilized sweetmeats (1:10) were used to prepare 80% methanolic extractions at room temperature for 24 hours for all the assays. Helapa was further investigated for the changes in AP with the changes of ingredients utilized in different regions of Sri Lanka. Accordingly, Helapa prepared with 100% finger millet flour, finger millet+rice flour, Shorea megistophylla (sin. Beraliya) flour+rice flour, Vateria copallifera (sin. Hal) flour+rice flour, Madhuca longifolia (sin. Mee) flour+rice flour was studied. Results revealed that, among sweetmeats examined for AP, Naran Kewum showed the significantly highest AP (1595.7±0.03 µg/mL TE) by FRAP assay and Helapa showed the highest radical scavenging activity for DPPH (564.8± 0.02 µg/mL TE) and ABTS (553.2±0.01 µg/mL TE) assays (p<0.05). Naran Kewum showed the significantly highest TPC (293.44 GAE/100 g) and TFC (237.8±0.34 QE/100g). In addition, rice flour mixed with Shorea megistophylla flour in preparation of Helapa had the highest TPC (316.9±0.28 GAE/100 g), the highest radical scavenging activity for DPPH (541.6±0.67 µg/mL TE) and ABTS (502.6±0.21 µg/mL TE) assays (p<0.05) with compared to other ingredients added Helapa. These AP may be due to stable polyphenolics at high temperatures and newly formulated molecules by Maillard reaction. It is apparent that, Sri Lankan traditional sweetmeats had significant AP which are related with positive health benefits. en_US
dc.language.iso en en_US
dc.publisher Uva Wellassa University of Sri Lanka en_US
dc.subject Food Science en_US
dc.subject Food Technology en_US
dc.title Comparison of ABTS, DPPH, and FRAP Assays for Estimating Antioxidant Potential of Selected Sri Lankan Traditional Sweetmeats en_US
dc.title.alternative International Research Conference 2019 en_US
dc.type Other en_US


Files in this item

This item appears in the following Collection(s)

Show simple item record

Search UWU eRepository


Browse

My Account