Antibacterial Activities of Endophytic Fungi of Cyperus iria Collected from Matale Distric

Show simple item record Jayasundara, J.M.N.M. Ratnaweera, P.B. de Silva, E.D. 2019-07-08T08:14:13Z 2019-07-08T08:14:13Z 2018
dc.identifier.isbn 9789550481194
dc.description.abstract Antibiotic resistance of bacteria has become an ongoing severe human health concern which requires extensive research priority. Endophytic fungi of Cyperaceae family plants are considered as a potential source for isolating bioactive compounds. Hence, the objective of the current study was to isolate endophytic fungi of Cyperus iria and investigate the antibacterial activities of the crude fungal extracts. Healthy C. iria plants were collected from Weragama in Matale district and endophytic fungi were isolated from the surface sterilized roots and aerial parts using five types of media (SYP, YPD, ME, PDA and MEA) enriched with antibiotics. Each pure fungal culture was sub cultured in ten PDA dishes, incubated close to sporulation, extracted into ethyl acetate, filtered and resulting crude extracts were obtained. The crude extracts were tested for antibacterial activity using agar disc diffusion assay against four bacteria, Staphylococcus aureus (ATCC 25928), Bacillus cereus (ATCC 11718), Pseudomonas aeruginosa (ATCC 9027) and Escherichia coli (ATCC 35218) at 400 ug disc-1 concentration where Gentamycin (10 ug disc-1) and methanol (10 IA disc') were used as the positive and negative controls, respectively. Total 34 morphologically distinct putative endophytic fungi, 23 from aerial parts and 11 from roots, were isolated. Thirteen fungal extracts exhibited antibacterial activity against S. aureus, 24 against B. cereus, 12 against P. aeruginosa and one against E. coli. Among all, 29 fungi were active against at least one bacterium tested while five fungi were inactive to all. Activity of three extracts against B. cereus, 12 against P. aeruginosa, and one against E. coli was similar to the activity of the positive control. Fifteen extracts against B. cereus, 27 against P. aeruginosa, 33 against E. coli and 21 against S. aureus showed significant (p < 0.05) antibacterial activities compared to the negative control. In conclusion, C. iria from Matale harbors a lot of endophytic fungi, where several are capable of producing bioactive secondary metabolites with selective antibacterial properties. en_US
dc.language.iso en en_US
dc.publisher Uva Wellassa University of Sri Lanka en_US
dc.subject Biotechnology en_US
dc.subject Bioprocess Technology en_US
dc.subject Bio Chemicals Engineering en_US
dc.title Antibacterial Activities of Endophytic Fungi of Cyperus iria Collected from Matale Distric en_US
dc.title.alternative International Research Conference 2018 en_US
dc.type Other en_US

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