Analysis of Bioactive Properties of Fish Protein Hydrolysates from Scomber japonicus Fin Wastes

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dc.contributor.author Ediriweera, T.K.
dc.contributor.author Aruppala, A.L.Y.H.
dc.contributor.author Abeyrathne, E.D.N.S.
dc.date.accessioned 2019-07-03T19:38:23Z
dc.date.available 2019-07-03T19:38:23Z
dc.date.issued 2019
dc.identifier.uri http://www.erepo.lib.uwu.ac.lk/handle/123456789/1383
dc.description.abstract Scomber japonicus is a salience fish species used in canned fish processing. Its wastes are discarded, causing economic losses and environmental pollution. Study aims on producing bioactive Fish Protein Hydrolysates (FPH) utilizing fish wastes as a remedy. First, Scomber japonicus fin waste was collected and blended. Aqueous extracts of fish protein were produced with 04 different ratios as sample: distilled water, 1:1, 1:2, 1:3, 1:4. Best extraction was selected using 10% Sodium Dodecyle Sulphate Poly Acrylamide Gel Electrophoresis. Extracts were hydrolyzed using Papain, Pepsin, Trypsin and Protease enzymes (1:100) at 37ºC under their optimum pH conditions for 0, 3, 6, 9, 12 and 24 hours followed by heat inactivation at 100ºC for 15 minutes. Hydrolysates were lyophilized and analyzed for antioxidant activities by Thiobarbituric acid reactive substances (TBARS) assay and diphenyl picrylhydrazyl (DPPH) scavenging assay, metal chelation activity by Fe (II) chelating activity and antibacterial activities by agar well diffusion method. No significant difference was observed among 04 ratios in yield (p>0.05). 1:1 ratio was selected for hydrolysis experiments. None of the FPHs showed antioxidant properties with TBARS assay (p<0.05). Radical scavenging activity demonstrated a significant difference among treatments (p<0.05). Fe (II) chelating activity revealed Fe releasing instead of chelation (1.84, 13.99, 16.48, 1.84 %,). Antibacterial activities against E.coli and Salmonella spp. were highly positive in all hydrolysates showing best activity against both strains by Trypsin hydrolysate. This concludes the FPHs of water extracted Scomber japonicus fin wastes contain strong antibacterial activity, weak antioxidant activity and iron releasing properties. en_US
dc.language.iso en en_US
dc.title Analysis of Bioactive Properties of Fish Protein Hydrolysates from Scomber japonicus Fin Wastes en_US
dc.type Article en_US


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